Material Requests

Material Requests

The Laboratory for Genomics Research (LGR) is dedicated to advancing drug discovery and academic research. In partnership with GSK, LGR has developed a genome-wide dual guide CRISPRi library, a CRISPRko guide library, and the LGR guide vector backbone plasmid. These resources will be made available to UC San Francisco and UC Berkeley investigators to further the field of functional genomics.

ITEM 1

Genome-wide CRISPRi Library

(Pooled Library #187246, #187247, #187248, #1000000192)

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PURPOSE
‍Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

VECTOR BACKBONE
‍pJR104 (Plasmid #187243) - Does not express Cas9

DEPOSITING LABS
‍Jonathan Weissman

‍PUBLICATION
‍Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.7554/eLife.81856.

Library Details
Genes/gRNAs/plasmids: 22,580 dual sgRNA elements
Controls: 1,026 dual sgRNA control elements
Lentiviral Generation: 3rd

Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
‍Volume: ∼20 µL
‍Concentration: 50 ng/µL

ITEM 2

CRISPRko "Brunello" Guide Library

(Pooled Library #187246, #187247, #187248, #1000000192)

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PURPOSE
‍Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

VECTOR BACKBONE
‍pJR104 (Plasmid #187243) - Does not express Cas9

DEPOSITING LABS
‍Jonathan Weissman

‍PUBLICATION
‍Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.7554/eLife.81856.

Library Details
Genes/gRNAs/plasmids: 22,580 dual sgRNA elements
Controls: 1,026 dual sgRNA control elements
Lentiviral Generation: 3rd

Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
‍Volume: ∼20 µL
‍Concentration: 50 ng/µL

ITEM 3

pU6-sgRNA EF1Alpha-puro-T2A-BFP

(Plasmid #60955)

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PURPOSE
Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

DEPOSITING LABS
‍Jonathan Weissman

‍PUBLICATION
‍Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.7554/eLife.81856.

Sequence information
Sequences (4)

Library Details
Vector backbone: pSICO derivative
Backbone manufacturer: Addgene
Backbone size w/o insert (bp): 8200
Total vector size (bp): 8888
Vector type: Mammalian Expression, Lentiviral, CRISPR
Selectable markers: Puromycin

Library Shipping
Bacterial Resistance(s): Ampicillin, 100 μg/mL
‍Growth Temperature: 37°C
‍Growth Strain(s): Stbl3
‍Copy number: Low Copy

Gene/Insert 1
‍Gene/Insert name: sgGFP-NT2
‍Insert Size (bp): 113

Gene/Insert 2
‍Gene/Insert name: puro-T2A-BFP
‍Insert Size (bp): 1371

Resource Information
‍Supplemental Documents
‍pU6-sgRNA EF1Alpha-puro-T2A-BFP
‍Articles Citing this Plasmid
‍61 References

‍Terms and Licenses
‍Academic/Nonprofit Terms
- UBMTA
- Evrogen Limited Use Label License for FPs

‍Industry Terms:
- Not Available to Industry

Trademarks:
- Zeocin® is an InvivoGen trademark.

46

Principal Investigators

31

Research Projects

52

Published Papers

112k

Samples Tested

Accomplishments To Date

January 2021: Built and validated core suite of CRISPRi/a cell lines
March 2021: Computational pooled screen analysis pipeline deployed
July 2021: First in-house FACS-based screen completed
August 2021: Arrayed Screening platform build completed
August 2021: Generation of transferable genome-wide CRISPRi library
October 2021: First single cell functional genomics screen completed

Our Roadmap

PAST

May 2019:
Collaboration agreement signed

October 2019:
Inaugural LGR symposium

April 2020:
First wave of university-funded projects to support Covid research

July 2020:
First collaborative project initiated

October 2020:
First genome-wide screen completed

PRESENT

August 2021:
SOMETHING ABOUT AUTOMATION EQUIPMENT / THERMO SYSTEM

FUTURE

[Date]
Transferable CRISPRi library

[Date]
Computational pipeline?

[Date]
Staffing %

Place An Order

Our Process:

After submitting an order through our form, we will contact you to confirm and approve your request. Once approved, a material transfer request will be initiated, and the recipient will coordinate the delivery.

An MTA is required to transfer these materials to UCSF and UCB labs.

‍

Materials Available:

- Genome-wide CRISPRi Library

- CRISPRko "Brunello" Guide Library

- Vector Backbone Plasmid: BFP, eGFP, mCherry

‍

*Please use the "other" request form when requesting multiple materials.
‍

For all other inquiries:

Please fill out the form as "other" and our team will get back to you on whether the request is possible.

* These materials were created by your colleagues. Please acknowledge the Laboratory for Genomics Research in publications or presentations when using these materials.

* Please note that the LGR only supports material requests for UC Berkeley or UC San Francisco.

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Place an order for materials created by our lab!

Material Requests

Genome-wide CRISPRi Library

CRISPRko "Brunello" Guide Library

pU6-sgRNA EF1Alpha-puro-T2A-BFP

Accomplishments To Date

Our Roadmap

Place An Order

Our Process:

Materials Available:

For all other inquiries: